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polyclonal antibody anti-cd63-biotin  (MyBiosource Biotechnology)

 
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    MyBiosource Biotechnology polyclonal antibody anti-cd63-biotin
    EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, <t>CD63,</t> MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.
    Polyclonal Antibody Anti Cd63 Biotin, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody anti-cd63-biotin/product/MyBiosource Biotechnology
    Average 90 stars, based on 1 article reviews
    polyclonal antibody anti-cd63-biotin - by Bioz Stars, 2026-02
    90/100 stars

    Images

    1) Product Images from "Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response"

    Article Title: Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2024.1440667

    EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, CD63, MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.
    Figure Legend Snippet: EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, CD63, MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.

    Techniques Used: Incubation, Labeling, Purification, Control, Comparison, Marker, Fluorescence



    Similar Products

    polyclonal antibody anti-cd63-biotin  (MyBiosource Biotechnology)
    90
    MyBiosource Biotechnology polyclonal antibody anti-cd63-biotin
    EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, <t>CD63,</t> MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.
    Polyclonal Antibody Anti Cd63 Biotin, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal antibody anti-cd63-biotin/product/MyBiosource Biotechnology
    Average 90 stars, based on 1 article reviews
    polyclonal antibody anti-cd63-biotin - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, CD63, MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.

    Journal: Frontiers in Immunology

    Article Title: Extracellular vesicles derived from antigen-presenting cells pulsed with foot and mouth virus vaccine-antigens act as carriers of viral proteins and stimulate B cell response

    doi: 10.3389/fimmu.2024.1440667

    Figure Lengend Snippet: EVs-FMDVi express FMDV proteins, classical EVs markers, and immunoregulatory molecules. EVs were coupled with aldehyde/sulfate latex beads of 4 µm diameter and incubated with different fluorochrome-conjugated monoclonal antibodies anti-CD9, anti-CD81, CD63, MHC-II, CD11c, CD86 as detailed in materials and methods. For viral protein detection on EVs, FITC-labeled IgG purified from an FMDV-immune bovine serum was used, and FITC-labeled IgG obtained from a healthy unimmunized bovine serum was used as a corresponding control (Control). Comparison of percentages for different marker proteins and FMDV viral proteins for each EV population. The mean value ± SEM of the Median Fluorescence Intensity (MFI) for each of the markers evaluated is also shown. For statistical analysis, the Kruskal-Wallis test followed by Dunn’s multiple comparisons test was used for the analysis of MFI, and one-factor ANOVA followed by Bonferroni’s multiple comparisons test for the analysis of percentages. Only significant differences are indicated in the graphs (p<0.05 *; p<0.01**; p<0.001 ***). The data correspond to 15 independent experiments.

    Article Snippet: After being saturated with 100 mM glycine, EVs were incubated with the following monoclonal antibodies: 0.02µg/100µl anti-MHC-II-PE (Biolegend-clone: M5/114.15.2), 0.2µg/100µl anti-CD11c-APC (Biolegend-clone: N418), 1.5 µg/100µl anti-CD86-FITC (Miltenyi-clone: PO3.3), 1 µg/100µl anti-CD9-biotin (eBioscience-clone: MEM61), 1 µg/100µl anti-CD81-biotin (eBioscience clone: Eat2) or 1 µg/100µl polyclonal antibody anti-CD63-biotin (MyBioSource).

    Techniques: Incubation, Labeling, Purification, Control, Comparison, Marker, Fluorescence